The HPLC Family: Rapid HbA1c analysis and the best variant detection available.

Tosoh's advanced HPLC systems are fully automated systems that rapidly and precisely separate haemoglobins found naturally in human blood.  Suitable for high throughput HbA1c testing in the laboratory or clinic environment in addition to rapid Haemoglobinopathy screening, our HPLC solutions provide a reliable, accurate and precise service at all times.

To discover more about our HPLC solutions please visit the following pages: 

                     G8 Analyser                       G7 Analyser                  G5 Analyser

The key applications of their analysis modes are as follows:

Follow-up of diabetic patients:

HPLC is considered the “Gold Standard” technology in the follow-up of the plasma glucose concentration of diabetic patients over time, via the measurement of HbA1c (= glycated haemoglobin fraction). This technique was used in the ground breaking DCCT (Diabetes Control and Complications Trial) study. 

To follow-up the plasma glucose concentrations over time the “HbA1c analysis mode” should be used. In this mode predetermined windows are set in the software to detect the presence of Haemoglobins A1a, A1b, F, l-A1c, s-A1c and A0. Calibration against DCCT (NGSP) or IFCC allows the quantitation of the stabile A1c fraction, with other windows allowing presumptive identification of various variant haemoglobins.

 Read more about diabetes management.

Screening for β-thalassaemia and haemoglobinopathies:

In the aid of diagnosing β-thalassaemia and haemoglobinopathies the “β-thalassaemia analysis mode” should be used. In this mode predetermined windows are set in the software to detect the presence of Haemoglobins F, A0, A2, D+, S+ and C+.  Calibration allows the quantitation of HbF and HbA2, with all other windows allowing the presumptive identification of various variant haemoglobins.

 Read more about haemoglobopathies.

High Performance Liquid Chromatography (HPLC)

 This technique is a form of column chromatography used frequently in biochemistry and analytical chemistry. It involves passing a mixture containing the “analyte” through a column (stationary phase), by a liquid (mobile phase) at high pressure. 

Cation exchange chromatography is a process that allows the separation of the mixture based on the charge properties of the molecules in the mixture. Cation exchange chromatography retains analyte molecules based on coulombic (ionic) interactions.

The stationary phase surface displays negatively charged functional groups that interact with positively charged cations in the mixture.

 Tosoh HPLC analyser separation Principle:

 The G5 (a.k.a A1c2.2), G7  and G8 analysers are fully automated systems that rapidly and precisely separate haemoglobins found naturally in blood. Charged haemoglobins and other haemoglobin components are eluted at varying times depending upon the net charge of the molecule in relation to a gradient of increasing ionic strength passed through a non-porous cation exchange column (negatively charged beads).